Nuclear Dna Amount Assessment In Some Representatives Of Tribe Anthemideae (Asteraceae)

M Sanz, S Garcia, T Garnatje, H Inceer, A Kreitschitz, ED Mcarthur and J Vallès

Laboratori de Botànica, Facultat de Farmàcia, Universitat de Barcelona, Av. Joan XXIII, s/n, 08028 Barcelona, Catalonia, Spain
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Abstract


    Using Pisum sativum cv Express Long and Petunia hybrida cv pcpx6 as internal standards, genome size was estimated by flow cytometry for the first time in 64 populations of 53 species or subspecies of tribe Anthemideae (Asteraceae). We also used the previous results obtained by our group(1) in 25 Artemisia populations for discussion. Most of the taxa considered belong to subtribe Artemisiinae (7 genera, 57 taxa), and the others to subtribes Matricariinae (1 genus, 9 taxa), Tanacetinae (3 genera, 3 taxa), Leucantheminae (1 genus, 1 taxon) and Handeliinae (1 genus, 1 taxon). The taxa studied represent distinct life forms, basic chromosome numbers, and ploidy levels in the tribe. The C values obtained range from 3.50 pg for the diploid Artemisia annua to 25.65 pg for the octoploid Artemisia cana. Our results show that variations in genome size have some systematic and evolutionary implications. In Artemisia, the most extensively-investigated genus, the greater variation observed in genome size within than between the sections or subgenera could be due to the fact that the present infrageneric classification does not represent natural groups. Several aspects, e.g. karyological (chromosome number, ploidy level, karyotype length), morphological (presence or absence of rhizomes) or ecological (adaptation to arid lands, among others), correlate well with nuclear DNA content.

    1. Torrell M, Vallès J (2001). Genome 44: 231-238.