Genome Size Variation In Echinops And Related Genera (Asteraceae, Cardueae)

T Garnatje1,2, S Garcia3, O Hidalgo1, M Sanz3, S Siljak-Yakovlev2 and J Vallès3

1 Institut Botànic de Barcelona-CSIC, Av. Muntanyans s.n., 08038 Barcelona, Catalonia, Spain
2 Departement d’Evolution et Systématique, UMR-CNRS 8079, Université de Paris-Sud, Bât. 360, 91405 Orsay Cedex, France
3 Laboratori de Botànica, Facultat de Farmàcia, Universitat de Barcelona, Av. Joan XXIII, s.n., 08028 Barcelona, Catalonia, Spain

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Abstract


    We studied 33 taxa of the tribe Cardueae (22 species of Echinops and 11 of other related genera) in order to discover any potential relationship between their genome size and several systematic, ecological and karyological parameters. Nuclear DNA amount for each species was measured by flow cytometry. The 2C-values ranged from 1.49 pg in Staehelina petiolata to 16.98 pg in Siebera pungens. In addition, three karyological parameters were obtained for each species: chromosome number, total length of chromosome set, and mean chromosome length. A phylogenetic analysis was carried out using the sequences of both the nuclear internal transcribed spacer region (ITS) and the chloroplast gene matK (unpublished data). Karyological results and those obtained in the molecular phylogeny analysis were compared with those from the DNA amount study. ANOVA and correlation analyses were also performed between genome size data and some ecological parameters (life cycle, habitat, distribution and altitude) to search for any significant relationships among these variables.